Blood homocysteine levels in children with autism spectrum disorder: An updated systematic review and meta-analysis
Outcomes of research on peripheral blood ranges of homocysteine (Hcy) in kids with autism spectrum dysfunction (ASD) are inconsistent, and conclusions from two earlier meta-analyses on this topic printed in 2012 are already outdated. Due to this fact, we performed an up to date systematic evaluation and meta-analysis to quantitatively summarize the peripheral blood Hcy knowledge in kids with ASD in contrast with wholesome controls (HC).
We searched PubMed, EMBASE, PsycINFO, PsycARTICLES, Internet of Science, and Cochrane Library databases from inception to September 2019 for eligible research, with no language restriction. Utilizing random-effects mannequin, we computed abstract statistics. Thirty-one research (3304 members together with 1641 instances) have been included. The pooled outcomes confirmed that the peripheral blood Hcy ranges have been considerably elevated in kids with ASD when in comparison with HC (Hedges’s g = 0.56, 95% CI = 0.36 to 0.76, P < 0.001).
By sensitivity analyses, we confirmed that our outcomes have been fairly strong. Moreover, no publication bias was noticed on this meta-analysis. In conclusion, our examine help the affiliation of elevated circulating Hcy ranges with ASD in kids, and the involvement of Hcy within the prevalence of ASD. Nonetheless, in view of the numerous between-study heterogeneity, the conclusions must be interpreted cautiously and extra investigation is required.
Concentrations of homocysteine in follicular fluid and embryo high quality and oocyte maturity in infertile girls: a potential cohort
Homocysteine is without doubt one of the elements of follicular fluid (FF), in order that any disruptions in its focus might have an effect on oocyte improvement. The goal of this examine was to find out the connection between FF homocysteine focus and embryo high quality, oocyte maturity, and being pregnant fee.
Oocytes and embryos of 44 infertile girls have been categorised into totally different teams primarily based on their maturity and high quality, respectively. FF homocysteine ranges, oocyte maturity, embryo high quality, and being pregnant standing have been measured. A big affiliation was noticed between the degrees of FF homocysteine and oocyte maturation fee (p = .00).
The focus of FF homocysteine was greater than 9.eight µm/L in girls with oocyte maturation < 80%. Many of the good high quality embryos belonged to homocysteine ranges < 9.eight µm/L. Decreased FF homocysteine concentrations can considerably enhance the oocyte maturation fee and embryo high quality. Growing older could also be an oblique issue contributing to decreased embryo high quality and oocyte maturation by way of growing FF homocysteine ranges. IMPACT STATEMENT
What’s already recognized on this topic? It has been demonstrated that homocysteine is without doubt one of the elements of follicular fluid (FF), however no data is accessible in regards to the hyperlink between its focus in FF and oocyte improvement.
What do the outcomes of this examine add? The info indicated that decreased FF homocysteine concentrations at a youthful age might remarkably enhance the oocyte maturity and embryo high quality of infertile sufferers present process assisted reproductive therapy (ART).
What are the implications of those findings for scientific observe and/or additional analysis? Based mostly on the findings and contemplating the benefit of measuring serum homocysteine and its direct correlation with FF homocysteine, homocysteine stage measurement is really useful in sufferers who’re candidates for infertility therapy to be able to estimate oocyte maturation fee, embryo high quality, and ART outcomes. Future research are recommended to analyze sufferers with PCOS, endometriosis, and male issue infertility.
Blood homocysteine levels in children with autism spectrum disorder: An updated systematic review and meta-analysis
Homocysteine induces mitochondrial dysfunction and oxidative stress in myocardial ischemia/reperfusion damage by way of stimulating ROS manufacturing and the ERK1/2 signaling pathway
Acute oxidative stress and mitochondrial dysfunction are essential for acute myocardial ischemia-reperfusion (AMI/R) damage, which can induce cell or mitochondrial membrane rupture and myocardial infarction. Plasma homocysteine (Hcy) expression ranges are positively related to danger of heart problems, and ERK1/2 exert anti-apoptotic and cardioprotective results on AMI/R damage.
Nonetheless, the exact molecular mechanism of motion underlying the results of Hcy and the ERK1/2 signaling pathway on mitochondrial dysfunction and oxidative stress in AMI/R damage stays unclear. Within the current examine, AMI/R damage fashions have been established in an animal mannequin handled with Hcy and in H9C2 cells that have been handled with hypoxia-reoxygenation. Mitochondrial perform and oxidative stress have been evaluated. The outcomes demonstrated that Hcy enhanced ERK1/2 protein expression ranges and oxidative stress, induced cytochrome c translocation and mitochondria dysfunction, and triggered cardiac dysfunction in rats with AMI/R damage.
Nonetheless, an ERK1/2 inhibitor successfully protected AMI/R damage rats from Hcy-induced cardiac dysfunction and oxidative stress. In conclusion, Hcy induced mitochondrial dysfunction and oxidative stress in AMI/R damage by way of stimulating ROS manufacturing and the ERK1/2 signaling pathway. An ERK1/2 inhibitor could also be an efficient new therapeutic technique for treating Hcy-induced cardiac dysfunction in sufferers with AMI/R damage.
Serum HCY focus was statistically in contrast between 24 wholesome canine and 29 canine with immunosuppressant-responsive enteropathy. Correlation analyses between serum complete protein, albumin (ALB), C-reactive protein (CRP), folate and cobalamin, and serum HCY focus have been carried out in immunosuppressant-responsive enteropathic canine.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Homocysteine (HCy) in serum, plasma and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Homocysteine (HCy) in serum, plasma and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Homocysteine (HCy) in serum, plasma and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Homocysteine (HCy) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of General Homocysteine (HCy) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitative sandwich ELISA for measuring Rat Homocysteine (HCY) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Rat Homocysteine (HCY) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Rat Homocysteine (HCY) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Homocysteine (HCY) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Mouse Homocysteine (HCY) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Mouse Homocysteine (HCY) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Mouse Homocysteine (HCY) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Homocysteine is an amino acid intermediate that is synthesized from methionine via S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH). Elevated levels of homocysteine can indicate a risk for numerous diseases, including vascular and heart disease. Our Homocysteine ELISA is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-homocysteine antibody are added to a plate coated with a homocysteine conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high homocysteine levels will bind to most of the antibody, which gets washed away and results in a low OD, while samples with low homocysteine levels will leave more antibody available to bind to the conjugate, producing a high signal.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Homocysteine (HCy) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Homocysteine (HCy) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A competitive ELISA for quantitative measurement of Rat Homocysteine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Homocysteine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Homocysteine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Homocysteine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Homocysteine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Homocysteine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Homocysteine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Homocysteine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Homocysteine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HCy protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HCy. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HCy in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HCy protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HCy. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HCy in the samples is then determined by comparing the OD of the samples to the standard curve.
Though, serum HCY focus was greater in immunosuppressant-responsive enteropathy, its prognostic worth stays unclear. Nonetheless, additional potential, large-scale research are warranted to raised examine the doable prognostic function of HCY in immunosuppressant-responsive enteropathic canine.
